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I originally wrote this post for my old blog—a post that humorously described the hierarchy of a scientific research lab. I wrote this as a second-year PhD student in February 2016, but I thought it would be fun to reflect on this post as a now post-doc.

The following text is my original post, while my commentary in the present day is in red 😉.

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I often reference how I have to “pop into lab” or “go out for lunch after lab” in many of my posts, but I have not had the chance (or the patience) to go into detail about my life in lab. I thought it would be fun to describe the usual hierarchy of a lab, so you could have at least some idea of what I have to deal with on a daily basis…

Before I became the dedicated PhD student researcher I am today (😅) , I was an overworked, overexhausted undergrad student, who was treated like a grad student during my first lab research experience. Not all was bad though, as I learned a lot, and had face-time with my boss every time I was in the lab, but as an undergrad student I could only spend so much time in the lab. My boss at the time didn’t understand this, and she kept pressuring me to stay longer, work more, etc. I was able to hold on for a year before moving on to my next experience!

I think PIs don’t understand the concept of work-life balance in general…most just want you to get your tasks done, no questions asked. If you speak up and say it’s too much, perhaps if they are the reasonable type you can reach a compromise to prevent burnout, but most scientists keep to themselves and work without question. This has frustrated me over the years because it’s a form of peer pressure that isn’t healthy. We need breaks, we need rest, and we need to prioritize our time so that we take care of ourselves and our work doesn’t suffer…

The undergrads in my current lab are more like these guys though …

They’re not annoying, but most of them work under a post-doc in my lab who likes to call them “minions”. In my lab, they tend to carry out smaller experiments and tasks, but don’t necessarily have a project on their own. In comparison, I would say I had it a lot better as an undergrad (if we’re talking about getting experience and training… if we’re talking about the preservation of my sanity, then perhaps it would be the opposite 😂).

When it was time for me to recruit and mentor my own set of students, I treated them with dignity and respect. I knew that if I wanted them to help me with my work, I had to take the time to carefully train them and of course, practice patience. It’s never the student who is incapable of comprehending information—it’s the teacher’s job to present the information in a clear way.

Next up on the lab hierarchy are usually research techs/lab assistants. Usually people that are at this stage are students that just finished undergrad, and are trying to keep busy while waiting to hear from the grad/medical school programs they applied to. I fell into this stage right after I graduated from undergrad a year early, and fortunately, it was in a lab that was very laid-back. I wasn’t paid (so that meant working evening shifts at a local outlet store for some $$), but it kept me attuned with the research world as I applied, which also helped with my interviews!

But not all research techs/lab assistants are students-in-waiting. In fact, I’ve come across many people who are older adults, or people who got a B.S. or M.S. in some science major and had to take a hiatus from lab work due to personal circumstances.

My sister completed her master’s degree, and is currently working as a research assistant/lab manager at an established institution. She’s a relatively fresh graduate compared to many research techs/lab assistants—I know a woman who has held this position in a lab for 22 years 😱!

In either case, they’re on a never-ending quest to prove that they’ve got what it takes.

Then there are the grad students, the PhD-type 😛. Their origin can be one of these two: a continuing masters student, or one who is constantly replying “No, I came straight from bachelors” to anyone who asks, “Did you get a masters first?“.

Compared to my years as an undergrad, I would say being a grad student is less intense. Shocked? Don’t be! I still have plenty of work, and with my qualifying exam coming up, I’ll probably be rethinking what I type here, but I am being completely honest when I say I felt more stressed/anxious/obsessive about my work as an undergrad than I do now. I view the work I do now as my job, and I don’t necessarily see myself as a student in the traditional sense. I do have classes I have to take, but unlike in undergrad where my focus was on class and doing well in class, now it’s to produce work in lab and just show your face in class.

Yeah…I spoke to soon 😂. My third and fourth years were the most intense of my PhD career. I felt an incredible amount of pressure in my fourth year, as I was in the middle of manuscript writing. A lot of doubt and negative feelings resurfaced, and it was a tough period in my life to get through. Whoever says the path to a PhD is fun and easy is delusional 🙃

Just above the PhD students, you have the post-docs. These guys and gals try very hard to make sure everyone knows that they are NO LONGER STUDENTS and that they do in fact have 100% earned the right to be called _____ , PhD. There is no denying the fact that they do deserve this, but it can get irritating when you have grad student vs. post-doc arguments debates. The PhD student doesn’t want to look stupid when he/she asks a question to the post-doc, and the post-doc wants to make sure that they give the right answer, and “look good” doing so. They also have added pressure to be even more independent than a student, and are often regarded as mini-bosses in the lab.

I’m currently six months into my position as a post-doc in a new lab, and I have to remind myself often that I do indeed have a PhD after my name. That yes, I am a doctor. It may depend on the lab environment, but given the smaller size of my current lab and the lack of available students to help carry out expansive experiments, I’m back to doing all of my work on my own. And this definitely makes me feel like I’m a student 😓.

Finally, we have the primary investigator (Pl). The head honcho. The big boss. The don…

I’ve come across three PIs in my overall experience so far (not including rotations), and while they all have their own quirks and personality traits, a couple things stay common for all. As a Pl, your biggest responsibility is making sure your lab has adequate finances. It’s what gives you the freedom to run your lab the way you want to. It’s no secret that funding is extremely tight, so labs can get competitive when it comes to raking in mon-ayyy. In order to make sure the lab is successful, a Pl needs to be assertive, exact, frank, and be able to compromise if necessary (especially when it comes to collaborations, as science is definitely a “it’s who you know” kind of industry)…

Yup, this PI description is still pretty accurate 😁

It’s kind of funny that as a grad student, I fall right in the middle of all these interesting characters! At times, it almost feels like I’m in a sitcom or a TV show with all the crazy interactions and dynamics that go on around me or involve me. I do love it though…

Ah, how naïve I was back then! The remaining 3.5 years after this post was written were packed with work, but I did find my footing. As I gained seniority, I became more comfortable with my surroundings, my experiments, troubleshooting protocols, and presenting data. I trained over 20 undergraduate and master’s students who sought research experience, and I’ve presented at conferences and published a first author paper in a well-known journal.

I wouldn’t say I love this life, but when results from experiments make sense, your data is validated by your colleagues, and you are recognized for all of the time and effort you put in, it’s hard not to give yourself a pat on the back and take pride in your work!

Sometimes I wonder why I chose this life, but I have to admit that eventually…the difficult times have the potential to become something worthwhile and rewarding.

The risk is in the wait, and the unavoidable unknown…